FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670273
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11450.036.15543442
ACGTCTG13950.035.1160315
TCGTATG12350.034.35864343
AGTCACG14800.031.23519128
CGTATGC14600.029.53600144
TATGCCG14650.028.4932746
CAGTCAC17050.027.51771427
CACGTCT19000.026.5088314
ACACGTC20250.025.04284313
TAGCATC18150.023.94941936
GGTAGCA20350.022.886234
CGGGACT11500.022.8170131
CGCTATG19450.021.81657629
CGCGAAT13700.021.4210821
GTCGCTA20100.021.2825427
ATGCCGT20700.020.66549947
CACACGT24300.020.58541112
GTATGCC21450.019.9429345
GTCTGAA25400.019.55809817
ATCGGAA27100.018.5905442
Produced by FastQC (version 0.11.2)