FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4896190
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG8650.041.47871443
CTCGTAT8700.041.2403342
TATGCCG9650.037.5375246
AGTCACG10050.036.3867628
ACGTCTG10350.035.66393715
CGTATGC11350.032.5230744
GTCACGA12350.030.16896629
CACGTCT12650.029.72499814
CAGTCAC13800.026.74849927
GTATGCC14050.026.51835845
ACACGTC15650.025.12906613
TCACGAT14450.025.0683130
ATGCCGT15250.024.65813847
CGCTATG11600.022.60264229
GTCTGAA18250.020.98195617
GTCGCTA13000.020.4334727
CACGATC18250.020.22667531
CCGTCTT18250.019.65977550
CACACGT19500.019.28353312
CGGGACT7600.019.0781631
Produced by FastQC (version 0.11.2)