FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10108801
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT156240.15455838926891527No Hit
GTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCA133250.1318158305816882No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC133240.13180593821166328No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG113350.11213001423215276No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG106410.10526470943487759No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTG101920.10082303529370101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC13450.037.44754829
CTCGTAT8350.037.184342
TATGCCG9150.035.81839446
CGGGACT28000.034.7668571
ACGTCTG15900.034.49757415
CACGTCT15900.034.28043714
TCGTATG8800.033.71470343
AGTCACA16100.032.35518628
CAGTCAC16650.031.28593327
CACACTT16950.030.3257531
ACACGTC18450.030.10342613
GCCAGTA23050.029.6529981
TCACACT17950.028.25191330
GTGGATA43250.025.8602811
ATGCGTG23150.025.7871886
CTACCCG36800.025.692816
GTCTGAA21850.025.10349717
CATGCGT23250.024.9338155
ATGCCGT11950.024.82756647
TTACGGG12550.024.7399088
Produced by FastQC (version 0.11.2)