FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11378666
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT22700.034.1950442
TCGTATG23050.034.1248243
GTCACGT23450.033.98378829
ACGTCTG28250.032.11694315
CGTATGC25150.031.13798744
TATGCCG26350.030.37456346
CACGTAG27950.028.88262731
ACGTAGA27800.028.66618332
AGTCACG28650.028.65860228
ACACGTC32850.028.5647513
CACGTCT33750.027.59859314
CGTAGAG29350.026.79943333
TCACGTA32050.025.08017530
CACACGT37750.024.30901312
CAGTCAC37050.022.81280127
GTATGCC36300.021.85858245
GTCTGAA45650.021.0844517
CGGGACT19400.020.9973051
ATGCCGT39050.020.40758947
ATCGGAA48050.018.8864772
Produced by FastQC (version 0.11.2)