FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7728725
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG10350.033.330943
ACGTCTG15100.032.44112815
CTCGTAT9300.031.15901442
TATGCCG13400.027.28922746
CACGTCT18000.027.21450214
CAGTCAC16800.027.10533727
CGGGACT15500.025.3951781
ACACGTC20450.024.46015713
CGTATGC14300.024.36535344
AGTCACC20200.022.37241728
CGCTATG27950.021.22949229
GTCGCTA28200.020.9188227
GTCTGAA24800.019.89169317
GGTAACT30550.019.2012369
GTATGCC18700.019.00140845
ATGCCGT18250.018.71382147
GCTATGA32450.018.6044330
ATGCGTG17200.018.0575496
CTACCCG22000.018.0393096
GTGGTAA33600.017.45814767
Produced by FastQC (version 0.11.2)