FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5666935
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT84980.14995760494870683No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC64130.11316522952883701No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG62130.1096359848842452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7100.039.35700242
TATCTCG7400.038.6938339
TCGTATG7300.038.27839343
ACGTCTG10550.036.29563515
TATGCCG7650.036.07582546
ACACGTC10950.035.59985413
CACGTCT11650.033.16469214
TAATATC9050.032.78273836
CTATAAT8950.032.76386333
CGTATGC8850.032.35359644
AGTCACT9450.031.3953928
CACTATA10350.029.33199131
GTCTGAA14350.027.40568517
CAGTCAC11200.027.10590427
ATGCCGT10500.026.94090847
CGGGACT17250.026.6173381
TCACTAT11700.026.53724730
ATATCTC12500.023.4587238
CATGCGT17550.023.3970455
GTATGCC11800.023.3881445
Produced by FastQC (version 0.11.2)