FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25185661
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT257040.10205807185286897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACT58850.026.7503451
ACGTCTG52300.024.6697815
CACGTCT61550.021.35459714
ACACGTC64300.020.92424813
AGTCACT56200.020.6254128
GTCGCTA107500.020.1854127
CGCTATG107450.020.0344329
ATGCGTG58400.019.7939136
CTACCCG75300.019.7506796
CATGCGT58350.019.0419435
ACATGCG59850.018.9103644
GGGACTA83750.018.4992962
CAGTCAC65550.017.73587227
GGTAACT124750.017.1202569
GCTGAGT88350.017.06375312
GCTATGA130550.016.80656830
TACCCGC104100.016.2388217
GTGGTAA134450.016.1672967
GTCTGAA82600.015.82903917
TGCGTGC74400.015.62665757
Produced by FastQC (version 0.11.2)