FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8117073
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG19000.043.9400843
CTCGTAT16900.043.0725142
TATGCCG21650.040.47413646
CGTATGC21350.040.3960244
ACGTCTG27950.038.87942515
CACGTCT30600.035.85062814
ACACGTC32650.033.9166513
CAGTCAC30550.033.4252227
AGTCACC31650.032.04532628
GTATGCC27550.031.93157445
ATGCCGT29700.029.968547
CACACGT37900.029.03638512
GTCTGAA39050.028.09293617
GTCGTAT4850.024.89606742
ATCGGAA47300.023.1992932
AGCACAC48700.023.02241110
CCAGTCA46550.022.89998426
GCACACG49400.022.27717611
GAGCACA51500.022.0386549
CCGTCTT39000.021.93758650
Produced by FastQC (version 0.11.2)