FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_4bs_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_4bs_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15192396
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24000.035.3608943
CTCGTAT23700.034.49854342
TATGCCG25850.033.63107346
ACGTCTG30100.032.77913315
CGTATGC28050.030.62407344
CGGGACT29350.027.9944061
CACGTCT36000.027.98187314
ACACGTC38150.026.31448613
CAGTCAC36800.024.9366127
ACTCAAT37100.024.7346435
GTATGCC38750.022.70226745
ATGCCGT40650.021.04708147
CGCTATG53650.020.76985729
GTCTGAA50400.020.19246717
AGTCACC46850.019.88176328
GTCGCTA56300.019.73108927
CTACCCG39850.019.3959886
CATGCGT31300.019.1821445
ATGCGTG33300.018.8590326
ACATGCG32750.018.3326764
Produced by FastQC (version 0.11.2)