FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8073815
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9650.023.59471744
CGGGACT12650.022.3791871
ACGTCTG13500.021.4652315
TCGTATG10650.021.3792545
CGTATGC11700.020.93501746
TATGCCG12050.020.61311148
ACACGTC17650.018.17725613
GTCGCTA24100.018.03613927
CGCTATG24750.017.56257229
CACGTCT18050.016.62769714
TATCTCG14000.016.26380241
TGCGAGT16250.015.49790511
CTTAAAC20800.015.4362071
ACATGCG16850.015.1536064
ACCTAGG15300.015.1074887
ACCTATA19650.015.1019686
CTATAAG17600.015.09311546
GGTAACT29900.014.5402869
CTACCCG16500.014.4298836
CATGCGT17200.014.2436775
Produced by FastQC (version 0.11.2)