FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8946219
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG21900.029.14191615
ACACGTC24450.026.52556413
CACGTCT27150.023.88766314
CTCGTAT10750.023.4270242
CAGTCAC27000.022.48740827
AGTCACA28850.021.52383428
GTCACAT28050.021.39978229
CGTATGC14200.020.6502844
CGGGACT13550.020.6341361
GTCTGAA33650.019.78654917
TCGTATG12600.019.43930443
CACACGT33500.019.3599212
GTCGCTA24850.018.04710227
CGCTATG24800.017.66627329
ACATGAG33950.017.27436432
CGCGAAT15850.017.2043551
TATGCCG16050.016.98066346
ATCGGAA40000.016.3040942
TCGGAAG42000.015.445383
GCACACG43000.015.32349611
Produced by FastQC (version 0.11.2)