FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8594911
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT138300.1609091705545293No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC112220.13056563354757253No Hit
GTGGATACCTAGGTACCCAGAGACGAGGAAGGGCGTAGCAAGCGACGAAATGCTTCGGGGAGTTGAAAATAAGCA104850.1219907919930759No Hit
CAAATATTCAAATGAGAACTTTGAAGGCCGAAGAGGAGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTAAG100870.11736014485781178No Hit
CGCGAATCCTCTTGAAAGAGAGGGGTGCCCTCGGGAACGCGGACACAGGTGGTGCATGGCTGTCGTCAGCTCGTG96010.11170563604439884No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG92920.10811048537908072No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT88040.10243270698207348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT10700.050.94119642
TCGTATG11000.049.55160543
TATGCCG11150.048.57559246
AGTCACG11750.047.85673528
CACGGCT12050.045.52011531
CACGTCT15500.042.5106314
ACGTCTG15350.042.47655515
ACACGTC16000.040.96631613
ATGCCGT13600.040.07850347
CGTATGC14150.039.00828644
CAGTCAC14500.038.78090727
CGGGACT20650.038.286431
GCTACAT14650.037.67716235
GTCACGG15150.036.43348329
GTCTGAA18600.034.86910217
CTACATC16200.033.85949736
GGCTACA16900.032.86510534
AGCACAC20900.031.52738810
GAGCACA21700.030.8420269
GCCGTCT17900.030.64343549