FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10111625
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG23700.043.08566315
CTCGTAT14550.041.0190342
TCGTATG14250.040.18792343
AGTCACT26000.038.21334528
CACGTCT27450.037.45099314
TATGCCG18500.036.17698346
CAGTCAC27650.035.43373527
ACACGTC30400.034.27085513
CGTATGC20250.032.19843744
TATCTCG21550.029.29585339
CTTATCT25250.028.96535937
CTTATCG8700.027.36114737
GTCTGAA38900.026.87118117
ATGCCGT25650.026.49605447
AGCACAC39900.026.2850910
GTATGCC26550.026.11763645
CGGGACT20800.023.4052141
GAGCACA46150.022.9496179
TTATCTC28350.022.87753538
CACACGT46000.022.49890712
Produced by FastQC (version 0.11.2)