FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6736453
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11700.036.56266844
TCGTATG12500.036.1545845
CGTATGC13300.034.75803446
GTCACGT13150.034.62956229
ACGTCTG15200.033.36382315
CACGTGA14050.032.41153731
TGAAACG14900.031.25716235
TATGCCG14950.029.76784148
ACACGTC18000.029.3236313
AGTCACG15550.029.28480328
CACGTCT18450.029.16936914
TCACGTG16250.028.87249830
GAAACGA16650.027.1432836
CAGTCAC20300.022.77233327
CACACGT22400.022.63990412
AAACGAT20850.022.33758237
GTATGCC21400.022.24679247
CGGGACT10400.022.2396141
GTCTGAA24450.021.72920817
ATGCCGT22950.019.3912549
Produced by FastQC (version 0.11.2)