FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16152424
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27400.035.75731744
TCGTATG29000.034.61732545
GTCACGT31400.032.9599129
ACGTCTG36250.031.69051715
CGTATGC32950.031.61923446
ACGTCCG33900.030.42766632
AGTCACG35600.029.26510228
TATGCCG35550.027.94812648
TCACGTC39850.026.05765330
CACGTCT44250.026.03903814
CGCACAT38400.025.96393437
CCGCACA39550.025.81941636
ACACGTC47700.024.37267913
GTCCGCA45450.022.4675134
CAGTCAC47800.022.15655527
CACACGT54900.021.1137512
GTATGCC52750.020.0123847
ATGCCGT51850.019.62786549
TTACGGG16500.018.6084968
CGGGACT31200.018.5882991
Produced by FastQC (version 0.11.2)