FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12072519
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGAAT19850.030.24029235
ACGTCTG23750.027.88877515
ACACGTC28800.023.83704413
CACGTCT32600.021.587614
AGTCACT29350.020.80465728
GTCACTC30050.020.54962729
ACTCCCG29400.020.18257332
CACACGT35550.019.40806412
GTCGCTA34150.019.29478327
CGCTATG35500.018.75531629
CGGGACT20950.018.2922571
CAGTCAC33050.018.26685727
CGAATCG19800.016.02969637
ATCGGAA41550.016.0289762
GGTAACT42400.015.705235569
GTCTGAA45950.015.09045717
TCACTCC42800.014.58915830
CCGAATC44900.014.21438336
CACTCCC43000.014.03993331
GTGGTAA48800.013.92839967
Produced by FastQC (version 0.11.2)