FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16473248
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT168340.10218992635817781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25950.032.03907442
TCGTATG28500.030.2615743
ACGTCTG33000.029.79343815
CGTATGC29900.029.19059844
TATGCCG31150.027.57631946
CGGGACT35150.027.5032331
CACGTCT38100.025.98627514
ACACGTC39800.025.22309113
GTCACTC34650.025.08951629
AGTCACT36000.024.0527628
CAGTCAC42400.020.7476727
CGCTATG70950.020.421729
GTCGCTA70800.020.4162427
CACACGT51600.019.32148412
ATGCCGT45100.019.27609347
CTACCCG46900.019.1296146
GTATGCC45900.018.78969245
ATGCGTG38850.018.2970876
GTCTGAA55350.017.95006217
ACATGCG39800.017.5132264
Produced by FastQC (version 0.11.2)