FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6450346
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13850.027.64796615
CTCGTAT9450.027.01490242
TATGCCG10800.026.51293646
TCGTATG10300.025.1202643
CGTATGC10850.024.80075844
ACACGTC16050.024.50303313
CACGTCT16000.024.36399514
TATCTCG13400.021.62592139
CGGGACT9500.021.4390091
CGGAATA16700.020.45120234
CACACGT19850.019.63848512
CGCTATG15600.018.35482629
CAGTCAC20150.018.1479527
GTATGCC16650.018.0263845
GTCGCTA15850.017.84766427
AGTCACC21100.017.82121828
ATCGGAA23650.016.1955742
GAATATC23350.015.21775136
GTCTGAA26000.014.7278617
GCACACG27850.014.49297111
Produced by FastQC (version 0.11.2)