FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir64_3bs_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir64_3bs_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14502062
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG30400.031.6614843
ACGTCTG34850.030.98401315
TATGCCG31150.030.89905746
CTCGTAT30800.030.80236442
CGTATGC31400.030.32344444
ACACGTC39550.027.9997813
CACGTCT40100.026.92740814
GTATGCC40550.024.1616545
CAGTCAC46500.022.40526427
CGGGACT23000.022.2155971
CACACGT52750.020.86247412
AGTCACC49950.020.65048828
ATGCCGT49300.020.22320647
GTCTGAA57100.019.27319317
GTCGCTA38600.018.3215727
CGCTATG38950.018.15699629
ATCGGAA65500.016.5417632
GCCGTCT59300.016.23112549
GCACACG68700.016.21988911
TCTCGTA59650.015.96249241
Produced by FastQC (version 0.11.2)