FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6185866
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG14650.053.69039533
GTCACGT17900.045.2905129
CACGTTT17750.044.8961931
AGTCACG18100.044.4088728
TTTCGGA17900.044.13522735
ACGTTTC18650.042.54464332
ACGTCTG20850.042.1916515
TTCGGAA19700.040.62858236
CTCGTAT10150.038.74701344
TCGTATG9400.038.53579745
ACACGTC23950.037.01859713
TCGGAAT21600.036.73563837
CAGTCAC22600.036.17727327
CACGTCT25500.035.0390214
CGTATGC12400.031.1601346
CACACGT28800.030.78506312
TATGCCG12750.029.76359748
GTCTGAA30400.029.27780317
ATCGGAA35800.024.769442
GAGCACA38200.024.1133679
Produced by FastQC (version 0.11.2)