FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6654662
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA76740.11531765249685108TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14850.051.57372345
CTCGTAT15500.049.63352644
GTCACGT18200.043.97628829
AGTCACG18300.043.7359828
CGTATGC18050.043.57723246
ACGTCTG19900.043.33967215
TATCTCG18450.041.13689841
TATGCCG19000.040.85365348
GCCTTAT20450.038.96941837
CACGTCT23700.036.39042314
ACACGTC23800.036.38247313
CAGTCAC22600.036.02540227
TCACGTG22400.035.88474730
CTTATCT23500.032.5904539
CCTTATC24900.031.72790138
GTATGCC25300.031.36240247
ATGCCGT25300.030.6806149
CACACGT29050.030.52008212
TTATCTC25500.030.44020740
GTCTGAA29500.029.7036917
Produced by FastQC (version 0.11.2)