FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9606718
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG97020.10099182676123104TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG22200.050.3490243
CTCGTAT22850.047.1055342
ACGTCTG28150.044.8535815
TATGCCG25150.044.5804446
ACTGACC26450.043.04103532
CGTATGC27400.041.42283644
AGTCACT28150.040.93174728
CACGTCT31950.039.9503714
CAGTCAC29700.039.1440427
ACACGTC34100.037.43169813
GTATGCC32200.035.3553245
ATGCCGT32950.034.02725647
GTCTGAA39550.031.66351517
CTGACCA37950.029.99829733
ACCAATC39200.028.162436
AGCACAC47250.027.45298210
CCGTCTT41450.026.9661850
GAGCACA51050.026.5583049
CAATCTC41250.026.42841538
GCCGTCT41950.026.31582549
Produced by FastQC (version 0.11.2)