FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695563
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG141670.11159016736792217TruSeq Adapter, Index 1 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG32150.048.93060743
CTCGTAT30950.048.04152342
ACGTCTG39150.043.9702215
TATGCCG36900.042.9125946
CGTATGC39600.040.50911344
CACGTCT44850.038.61278514
AGTCACA41950.038.24013528
CAGTCAC42350.037.96026227
GTCACAT43250.037.25010329
ACACGTC47550.036.7106113
ATGCCGT46750.034.31387347
ATCACGA48550.033.46776234
GTATGCC48300.033.21270445
TCACGAT49050.032.84527235
GTCTGAA54800.031.79074317
CACACGT60800.028.54035812
CACGATC52400.028.17804736
CCGTCTT57650.027.88592550
CCAGTCA60250.026.9113726
GCCGTCT59950.026.64343549
Produced by FastQC (version 0.11.2)