FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5955202
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA89210.14980180353244105TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT15900.052.50567644
TCGTATG16900.051.84879745
ACGTCTG19600.046.6420715
TATGCCG19400.045.16724848
AGTCACA21000.042.0544928
CAGTCAC22650.039.4475127
CACGTCT23350.039.44652614
CGTATGC22800.038.88570446
GTCACAG22850.038.4986629
CACAGTC23400.037.88863431
GTATGCC23700.037.8457147
ACACGTC24800.037.41869413
ACAGTCA24050.036.7214832
GTCTGAA25400.036.12733517
TCACAGT24850.035.53930
ATGCCGT24950.035.12002649
GCCGTCT28700.030.89229651
CCGTCTT28800.030.4259352
GTCAACA29400.030.27385135
CAATCTC28550.029.48374640
Produced by FastQC (version 0.11.2)