FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6884200
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG17800.045.3524843
CTCGTAT15800.045.19792642
TATGCCG19000.042.85125746
ACGTCTG24500.041.3971715
CGTATGC20200.040.9887944
CACGTCT27500.037.63379314
ACACGTC27300.037.27794313
CAGTCAC26300.035.15400327
TGTAATC22100.034.1864436
TTGTAAT26500.033.5873635
TAATCTC21950.033.4772938
GTAATCT22000.033.40120337
GTATGCC25750.031.88635645
CTTGTAA28450.031.52776134
ATGCCGT25950.031.24177647
GTCTGAA33650.031.16577517
CACACGT34150.029.8007412
AGTCACC31950.028.9374128
ATCGGAA36550.027.7537672
CCAGTCA36900.026.45792626
Produced by FastQC (version 0.11.2)