FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15789798
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT206710.13091364436707803No Hit
CGGGACTACCCGCTGAGTTTAAGCATATAAATAAGCGGAGGAGAAGAAACTTACGAGGATTCCCCTAGTAACGGC158750.10053960158325015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT31750.046.39660642
TCGTATG34300.043.34906843
TATGCCG36450.040.9814246
CGTATGC37350.039.62445444
ACGTCTG43550.038.9731315
ACACGTC49050.034.8140513
CACCTAT44350.034.4594331
CACGTCT50600.033.88396514
CAGTCAC46450.033.3471427
ATGCCGT47600.032.1065347
AGTCACC48350.031.60859928
CGGGACT40050.031.5495341
CTATACA49300.030.99950834
TCACCTA50450.030.4980230
GTCTGAA58450.029.45129217
GTATGCC51100.029.43487445
GCCGTCT57650.026.27010549
TATACAT61250.024.89503735
TACATCT61200.024.29569437
CATCTCG62900.024.24244739
Produced by FastQC (version 0.11.2)