FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7865005
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT81100.10311500119834635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT16450.041.73434442
TATCTCG17400.039.85202839
TATGCCG18250.039.5071846
CGTATGC18050.039.18043544
TCGTATG17900.039.12405843
ACGTCTG21350.038.13326315
CACGTCT24350.034.00181614
ACACGTC25550.032.53988313
CAGTCAC23500.031.85570527
CTATCTC23050.030.08352938
CGGGACT16750.029.887031
AGTCACC24950.029.58937828
TCACCTA25450.029.00842330
GTATGCC25000.028.56407745
GCTATCT24850.028.32074537
ATGCCGT25350.028.03379247
GTCTGAA30350.027.50703417
CACCTAG27750.026.47980131
CACACGT35100.023.78487612
GCCGTCT30300.022.88474549
Produced by FastQC (version 0.11.2)