FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6162590
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT12750.048.43322842
TCGTATG14050.047.3894443
ACGTCTG18200.042.6482415
TATGCCG16500.040.97940446
CGTATGC16500.040.97907344
CACGTCT21300.036.927114
CTAATCT17400.036.481237
ACACGTC21200.036.45068413
CAGTCAC19350.036.36965627
TAATCTC18300.034.31001338
AGTCACC21050.033.59632528
GTATGCC20650.032.7435745
ATGCCGT21150.031.6432747
GTCTGAA26900.029.62440917
CACACGT28650.027.21349712
GAGCACA32800.025.1388729
CCGTCTT27200.024.8586350
AGCACAC32800.024.40258610
GCCGTCT30000.022.99845749
GCACACG34900.022.63767211
Produced by FastQC (version 0.11.2)