FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_4bs_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_4bs_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10239238
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG193600.18907656995569397RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG39750.053.80849543
CTCGTAT39250.053.52738642
TATGCCG42950.050.0404446
CGTATGC43250.049.93214444
ACGTCTG47850.048.59234615
CAGTCAC49650.044.5380127
CACGTCT52750.044.01315314
ACACGTC53500.043.5893813
ATGCCGT52700.041.04453347
AGTCACC54550.040.34781628
GTATGCC54200.039.90822245
CACACGT60450.038.86358312
GTCTGAA61550.037.9448317
GCCGTCT62300.034.11073349
CCGTCTT64950.032.7721150
AGATCTC65500.032.5497538
ATCGGAA72450.032.1971172
CCAGTCA70000.032.18156426
AAAGATC67600.031.79344636
GCTTGAA67050.031.69609359
Produced by FastQC (version 0.11.2)