FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_9_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_9_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11215135
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA159720.14241469228859038TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG34200.047.006445
CTCGTAT34650.045.69878844
CGTATGC37050.043.6698646
AGTCACG38250.042.3896428
ACGTCTG40900.041.83614715
TATGCCG38600.041.55878448
CACGAGT39950.040.58600631
TATCTCG39900.039.51339741
GTCACGA42050.038.80524429
TCACGAG44900.036.342130
CACGTCT49150.035.30512214
ACACGTC51000.034.15973713
ACGAGTG48000.033.77939232
GTATGCC48600.033.433547
CAGTCAC50900.032.3295527
CACACGT53450.032.20683712
GGATATC49150.031.93661738
GTGGATA51900.031.10825736
ATGCCGT54050.029.93465849
ATATCTC53750.029.90945240
Produced by FastQC (version 0.11.2)