FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_9_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_9_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6051867
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC12250.041.116938
TACGTAA13200.039.72585736
CTCGTAT12700.038.30146844
CGTATGC13900.037.72527346
ACGTAAT14000.037.70222537
TCGTATG14250.036.31419445
ACGTCTG17300.034.8973115
TATGCCG15050.033.925748
CCGTACG16100.032.9988233
TAATCTC17750.028.57081240
ACCGTAC19100.028.35762832
ACACGTC21800.028.16848613
CACGTCT21950.027.66165514
GTAATCT18500.027.22605539
CAGTCAC21150.025.93524627
GTATGCC21050.024.58344747
AGTCACC22500.024.53245728
CACACGT24900.024.24632312
GTCTGAA27400.022.28551717
ATGCCGT22850.022.19395649
Produced by FastQC (version 0.11.2)