FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_6_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_6_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8940687
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT20000.048.64239542
TCGTATG21250.046.26811243
ACGTCTG31750.044.98316215
CACGTCT35350.040.89008314
ACACGTC36450.039.9400213
TATGCCG26050.039.73004546
CAGTCAC34250.039.3830727
CGTATGC28850.036.23222744
AGTCACC40100.033.55200628
CACACGT43350.032.9463312
GTATGCC33150.032.26107445
GTCTGAA44900.032.03948617
ATGCCGT33050.030.89767647
AGCACAC51050.028.72078310
ATCGGAA51250.028.143642
CCAGTCA50950.026.94835526
TCGGAAG53650.026.6918493
GCACACG55000.026.59523611
TCTGAAC55450.026.50353818
CGTCTGA55100.026.35884316
Produced by FastQC (version 0.11.2)