FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_6_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_6_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13291164
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29950.048.49273343
CTCGTAT29900.048.45863742
AGTCACG32200.046.71148728
ACGTCTG35950.045.48476815
TATGCCG34750.042.2909946
GTCACGC37700.040.26283629
CGTATGC38850.038.00544444
TATCTCG38900.037.8679139
CACGTCT44200.036.99482314
GCCAATA42850.035.1020134
CAGTCAC43000.034.9795627
ACACGTC47300.034.8622113
CGCCAAT45450.033.3975933
CACGCCA45950.032.9588531
GTATGCC46250.032.2229345
ATGCCGT47100.031.3484447
CCAATAT50200.029.82501835
ATATCTC49900.029.72767638
TCACGCC51900.029.37974230
GTCTGAA58250.028.1313617
Produced by FastQC (version 0.11.2)