FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6660211
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG24950.047.01183315
CTCGTAT17050.044.31138644
TCGTATG16750.043.45734845
CAGTCAC28300.039.984327
AGTCACA29000.039.1378328
CACGTCT31400.037.57439814
TATGCCG19000.037.40379748
ACACGTC32100.037.18490213
CGTATGC20800.035.16208646
CACACGT33650.034.9612
CACATGT34050.033.3332531
GTCTGAA36050.032.91940717
ATCGGAA38500.030.3844872
GTATGCC24700.030.02918847
GTCACAT38950.029.40555829
CCAGTCA39400.029.33282526
ATGTCAG41200.028.21813234
CGTCTGA42250.028.0886316
TCCAGTC43250.027.27985625
TCGGAAG42950.027.2361833
Produced by FastQC (version 0.11.2)