FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_1_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_1_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9425012
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA98440.1044454903611794TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT20100.054.06482739
TCGTATG22950.046.4487345
CTCGTAT22950.045.99752844
TTCCGTA26650.043.10672436
ACGTCTG28900.041.5410215
CGTATGC26550.041.44996646
TATGCCG26700.040.8294848
TATCTCG27700.038.73298641
ACACGTC33800.035.82499713
AGTCACA32800.035.65490328
CACGTCT34600.035.19589614
CAGTCAC33250.034.86109527
GTCACAG33800.034.39589329
TCCGTAT33550.034.13861537
CCGTATC33350.033.7226838
GTATGCC34350.032.23861347
TCACAGT36700.032.1479630
AGTTCCG36900.031.41277734
ATGCCGT35000.030.75279649
CACAGTT39900.029.31029931
Produced by FastQC (version 0.11.2)