FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_12_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_12_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3657456
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG54910.15013167622522322RNA PCR Primer, Index 41 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG12550.054.42696843
CTCGTAT12250.054.07018342
TATGCCG13150.053.25531446
AGTCACG13600.053.01660528
ACGTCTG15350.049.66934615
GTCACGA14800.048.7179629
CGTATGC14300.048.0075744
ATGCCGT14650.046.62515347
CAGTCAC15550.046.58944727
CACGTCT16650.045.997214
ACACGTC16650.045.79000513
GTATGCC15750.044.2449345
GTCTGAA17800.042.83283617
ACGACAT17750.040.62006835
AGCACAC20700.037.8320710
GCCGTCT18800.035.782449
CACACGT21650.035.53363412
GAGCACA23350.033.3907439
CCGTCTT20500.033.15209650
CATCTCG20550.033.0709839
Produced by FastQC (version 0.11.2)