FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_12_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_12_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25092420
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT264930.10558168562458303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51600.042.05275744
TCGTATG55750.041.76917645
CACGTGA58500.040.3362531
GTCACGT59900.039.6237929
TATGCCG58600.039.50233548
AGTCACG60650.039.07676328
ACGTCTG68550.037.89447415
CGTATGC62150.037.801246
TCACGTG63050.037.7536830
ACGTGAT70950.033.4527132
ACACGTC80550.032.80580513
CACGTCT80150.032.75432214
CAGTCAC79100.030.39821827
GTATGCC80100.029.76083647
CGGGACT54650.028.9314461
ATGCCGT82450.028.2011749
CGTGATC87300.027.26659833
GTCTGAA96700.027.11313817
CACACGT110500.023.82091312
AGCACAC116050.022.83087510
Produced by FastQC (version 0.11.2)