FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_11_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_11_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9177649
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG113190.12333223900805099RNA PCR Primer, Index 31 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27550.047.33386242
TCGTATG29400.045.4112143
CGTATGC30150.044.73926544
TATGCCG31550.043.3004946
TATCTCG30650.042.5464339
ACGTCTG35050.042.42152815
ACGATAT35600.039.5373235
CACGATA36850.039.03893734
ACACGTC39300.037.83395813
CACGTCT40900.036.60694514
CAGTCAC39550.036.11214427
GTATGCC39050.035.33732645
CCACGAT40800.035.17487333
CGATATC38850.035.0756336
CACACGT45200.032.7428112
ATGCCGT41200.032.6561147
AGTCACC43400.032.59068728
ATATCTC44650.029.8241238
ACCACGA48450.029.76335732
GTCTGAA50300.029.62887617
Produced by FastQC (version 0.11.2)