FastQCFastQC Report
Fri 2 Mar 2018
HJJT3BGX5_n01_ir108_3bs_11_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJT3BGX5_n01_ir108_3bs_11_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15303666
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG46450.039.5116715
ACACGTC57350.032.8437713
CACGTCT56500.032.78846414
CAGTCAC56550.030.25838527
ACCGGAT57200.028.40656335
AGTCACC60200.028.13724928
CACACGT69200.026.82078212
GTCTGAA69750.026.6096417
ATCGGAA81300.022.8349722
CCAGTCA76950.022.68535826
AGCACAC83500.022.47555710
TCGGAAG84850.021.7570173
GCACACG86150.021.74416211
CGTCTGA86900.021.63603216
GAGCACA97900.020.0858529
TCCAGTC93600.019.90307625
GATCGGG59250.019.85473639
CGCTATG40950.019.62902329
TCTGAAC95900.019.53359218
CGGGACT26500.019.1510751
Produced by FastQC (version 0.11.2)