FastQCFastQC Report
Fri 15 Jun 2018
HJJNMBCX2_l02n02.332000000e7bf0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l02n02.332000000e7bf0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192016477
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG3501920.18237601557495506No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3315800.17268309739897997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA2611690.13601384843655892No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2046730.10659137340593952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG5428250.067.003432
AAGCAGT5569450.065.526911
CAGTGGT5569850.065.071444
AGTGGTA5713150.063.3924755
GTGGTAT6019800.060.2085466
GCAGTGG6045100.060.099923
GGTATCA6167600.058.8947568
TGGTATC6260850.057.9050067
GTATCAA7860300.046.564569
CATGGGG3405350.033.56347324-25
AGAGTAC6064300.032.9629918-19
GCAGAGT6175350.032.50968616-17
TACATGG6112650.032.2291622-23
ACGCAGA6558300.031.02298514-15
CAACGCA6574350.031.00944512-13
ATCAACG6657550.030.66334710-11
CGCAGAG6218000.029.25820716-17
ACATGGG6104900.028.90762724-25
AGTACAT6897600.028.85154320-21
CAGAGTA6257900.028.72713318-19
Produced by FastQC (version 0.11.2)