FastQCFastQC Report
Fri 15 Jun 2018
HJJNMBCX2_l02n01.331000000e7bf3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l02n01.331000000e7bf3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192016477
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG574350.018.1526281
TTGAACG640050.018.1457631
ACGAGGA540100.018.071361
CATGGCG569650.017.9941311
GCGCGAT503800.017.9771041
CTGCGGA409750.017.9331821
CTCGAAA504850.017.891811
AGAAACC594800.017.8100938
AGCTACC477750.017.7235158
GACTAAC462100.017.71521
TGACGGC407800.017.6785681
CTAAGAC610100.017.6714971
TGGCCAG681350.017.6091671
TGAGCCG511650.017.52661
GGGCATC556750.017.519591
TGGACGC534600.017.5039371
TGACTAG548750.017.4856511
ACGAGCC567700.017.4520991
GAATGAA449750.017.4488071
GACTGCG498850.017.4366871
Produced by FastQC (version 0.11.2)