FastQCFastQC Report
Fri 15 Jun 2018
HJJNMBCX2_l01n02.332000000e7be3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l01n02.332000000e7be3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193394461
Sequences flagged as poor quality0
Sequence length99
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG3544350.18327050225083746No Hit
GTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCTGGTGCAATTG3343290.17287413417698658No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA2633030.13614815989998805No Hit
GCTAAATAAACAAGAAAGCAAAATCAACAACCGCAAAAGTCTAAGACATG2074790.10728280372000934No Hit
GGTGCATATGGTGCCAACCCCCCTGGCCCCCCCAAAAGGGGGGTGGTGCT1942980.10046720004043962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG5481850.066.9493262
AAGCAGT5618950.065.4867251
CAGTGGT5617600.065.065814
AGTGGTA5753000.063.5793575
GTGGTAT6055450.060.43116
GCAGTGG6099150.060.080823
GGTATCA6203500.059.074578
TGGTATC6296000.058.0771147
GTATCAA7910450.046.6630489
CATGGGG3432750.033.61689424-25
AGAGTAC6092300.033.06717318-19
GCAGAGT6189700.032.54138616-17
TACATGG6154700.032.29476522-23
CAACGCA6594300.031.06007812-13
ACGCAGA6589350.030.9572614-15
ATCAACG6692050.030.68466210-11
CGCAGAG6238500.029.29833216-17
ACATGGG6127100.029.03954924-25
AGTACAT6940100.028.92552220-21
CAGAGTA6278050.028.84850118-19
Produced by FastQC (version 0.11.2)