FastQCFastQC Report
Fri 15 Jun 2018
HJJNMBCX2_l01n01.331000000e7be6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJNMBCX2_l01n01.331000000e7be6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193394461
Sequences flagged as poor quality0
Sequence length27
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG644000.018.2275561
ACGAGGA530950.018.1109731
GCGCGAT514350.018.0884461
CATGGCG566300.018.0608561
ACTGATG586900.018.0323641
CTCGAAA510850.017.9818921
CTGCGGA410050.017.8054181
CTAAGAC613600.017.7497041
TGGCCAG682250.017.7172161
AGAAACC597250.017.7065098
GGGCATC565150.017.6958121
TGAGCCG518450.017.6574611
GACTAAC466000.017.6415821
AGCTACC483700.017.6337558
TGACGGC411450.017.5889741
GATGCTA539500.017.4907381
ACGAGCC567500.017.4576641
TGACTAG560100.017.4574491
TGGACGC533850.017.4552961
GAATGAA457350.017.4511571
Produced by FastQC (version 0.11.2)