FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_nlp7_5_pchx_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_nlp7_5_pchx_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20969265
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA975300.4651092920996516TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC273550.057.6600547
TCTCGTA255200.057.101843
CGTATGC272600.056.98789646
CTCGTAT255800.056.9814844
TCGTATG259250.056.74204345
TTCCGTA320850.056.04179436
TCCGTAT323200.055.0899237
TATCTCG278250.054.380141
CGTATCT286900.054.2197239
CACAGTT358100.050.90626531
GTTCCGT354750.050.8713235
TCACAGT362850.050.23059530
CCGTATC346900.049.62537838
AGTCACA370900.049.1217928
CTTGAAA262100.048.67528562
TGCTTGA249100.048.50103860
TGAAAAA336000.048.45273264
ATCTCGT307700.048.3008542
AGTTCCG384900.047.15547634
GTCACAG387300.047.11301829
Produced by FastQC (version 0.11.2)