FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_nlp7_5_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_nlp7_5_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25269159
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC52700.022.51902245
CGTATGC55000.021.70272444
TCGTATG56700.021.05194343
CTCGTAT52600.019.93824642
TCTCGTA50100.019.76256641
AGTCACT72900.016.23272728
TGAAAAA87050.013.83126262
TGCTTGA86350.013.58375958
CTTGAAA89600.012.93713660
ACCAATC86200.012.16673536
GCTTGAA107400.010.82509259
GAAAAAA116050.010.70194163
CAGTCAC116250.010.5354827
CCAATCT106650.010.09256437
GTCACTG124500.09.97603829
GACCAAT121400.09.91785835
TATGCCG128650.09.78781246
ATCTCGT109100.09.61275840
TCACTGA137800.08.98814730
CAATCTC116450.08.97645538
Produced by FastQC (version 0.11.2)