HJJJKBGX5_n01_nlp7_180_pchx_r2.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HJJJKBGX5_n01_nlp7_180_pchx_r2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16459724
Sequences flagged as poor quality	0
Sequence length	75
%GC	61

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG	170151	1.0337415135272012	RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGG	33635	0.20434729039198957	RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG	31354	0.19048922083991202	RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG	17773	0.10797872430910749	RNA PCR Primer, Index 17 (100% over 43bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	45630	60.688095	43
CTCGTAT	42500	60.408974	42
GTATGCC	46140	59.972603	45
TCTCGTA	42505	59.825603	41
CGTATGC	46450	59.691193	44
ACGTAGA	48645	58.092564	32
GTAGAGA	48015	58.0637	34
CACGTAG	48770	57.8731	31
GAGATCT	43725	57.80238	37
AGATCTC	43680	57.774696	38
TAGAGAT	47755	57.627617	35
TCACGTA	49220	57.407246	30
CTTGAAA	46375	57.229042	60
TGAAAAA	48510	56.986046	62
GAAAAAA	49060	56.642532	63
CGTAGAG	50190	56.31117	33
TCTGCTT	46190	56.143383	56
TGCTTGA	46795	55.96324	58
TTGAAAA	48240	55.710213	61
AGTCACG	50930	55.42575	28