FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_nlp7_180_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_nlp7_180_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25674235
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC48200.021.40051345
TCGTATG48850.019.56212843
CGTATGC52550.019.1038644
ACACAGT100100.014.26846632
CACAGTG103500.013.79974433
AGTCACA104300.013.36341328
GTCACAC108900.013.11566329
TGAAAAA88600.013.04413662
CAGTGAT113250.012.58110135
CTCGTAT35450.012.45645942
AGTGATG73500.011.82821436
GAAAAAA101150.011.80088263
TCTCGTA37150.011.7007241
ACAGTGA122250.011.68321834
TCACACA124800.011.47232130
GTCGTAT57850.010.4956742
CTTGAAA79400.010.47134660
CAGTCAC135850.010.4122327
TGCTTGA82050.09.79670258
TGTCGTA60350.09.66073941
Produced by FastQC (version 0.11.2)