FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_input_nlp7_5_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_input_nlp7_5_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20782687
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGGG23050.051.63583449
GCGGGGT29800.049.89592750
GCATCGG32800.046.59442538
CATGGCA206650.045.242834
CATGGGG123950.045.08920739
ACCATGG212950.044.2934632
GTGTGGG26550.043.26979455
CCATGGC234100.040.27678733
GGGTGTA19850.039.62602659
TGGCATG177450.037.79440336
GGCATGG169600.037.59095437
ATGGGGG141400.036.84075540
GCATGGG160000.036.3315638
AGTCACC273750.034.58238628
CAGTCAC278150.034.09702327
TGGGTGT21500.033.8570558
GGGGTTT53600.033.1469443
GGCGGGG26200.032.65528549
GCCGGGT13600.032.46946750
TGGCGGG30350.032.2821748