FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_input_nlp7_180_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_input_nlp7_180_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26769231
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG400470.14960086077930293RNA PCR Primer, Index 35 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT137900.062.14306349
CGTATGC170950.061.93456644
TATGCCG170950.061.59149646
GTATGCC175450.061.58482445
TCTCGTA164200.060.08933341
CCGTCTT148450.059.6090950
ATCTCGT180400.058.74747540
GCTTGAA152800.055.60916559
TGCTTGA147150.055.2357658
GCGTGAA32850.051.5645859
CATTTTA297250.051.4502734
TATCTCG218900.050.62160539
ATGCCGT196450.049.80346347
GCGTATG29000.049.25007243
TCGTATG210050.048.434743
CTCGTAT206450.048.27665342
CTGCGTG22700.048.0249157
ATGCCGG26700.046.6445947
GCCGTGT15400.045.6998549
TGCCGTG20650.045.1075348
Produced by FastQC (version 0.11.2)