FastQCFastQC Report
Fri 2 Mar 2018
HJJJKBGX5_n01_input_bzip1_5_pchx_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHJJJKBGX5_n01_input_bzip1_5_pchx_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27456500
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG687080.25024311183144243RNA PCR Primer, Index 38 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT179950.062.45956449
ATGCCGT191000.061.9345647
CGTATGC195050.061.79842844
TGCCGTC187650.061.75332648
TATGCCG194950.061.4936846
GTATGCC196300.061.4222645
CCGTCTT186500.060.48802650
TCTCGTA179500.060.060141
ATCTCGT182800.059.50428840
TGCTTGA182950.058.34305658
GCTTGAA188200.058.0905559
TAGCTAT220250.056.7186335
CTAGCTA227400.055.95306834
CTTGAAA197950.055.6826460
ACCTAGC232950.054.67943632
TATCTCG227650.048.40266839
TCGTATG264350.045.7674743
TTCTGCT224250.045.644255
TGTCGTA17100.045.59453641
TGAAAAA274250.044.3170962