Basic Statistics
Measure | Value |
---|---|
Filename | HJJJKBGX5_n01_input_bzip1_5_pchx_r1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 27456500 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 68708 | 0.25024311183144243 | RNA PCR Primer, Index 38 (100% over 43bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTCT | 17995 | 0.0 | 62.459564 | 49 |
ATGCCGT | 19100 | 0.0 | 61.93456 | 47 |
CGTATGC | 19505 | 0.0 | 61.798428 | 44 |
TGCCGTC | 18765 | 0.0 | 61.753326 | 48 |
TATGCCG | 19495 | 0.0 | 61.49368 | 46 |
GTATGCC | 19630 | 0.0 | 61.42226 | 45 |
CCGTCTT | 18650 | 0.0 | 60.488026 | 50 |
TCTCGTA | 17950 | 0.0 | 60.0601 | 41 |
ATCTCGT | 18280 | 0.0 | 59.504288 | 40 |
TGCTTGA | 18295 | 0.0 | 58.343056 | 58 |
GCTTGAA | 18820 | 0.0 | 58.09055 | 59 |
TAGCTAT | 22025 | 0.0 | 56.71863 | 35 |
CTAGCTA | 22740 | 0.0 | 55.953068 | 34 |
CTTGAAA | 19795 | 0.0 | 55.68264 | 60 |
ACCTAGC | 23295 | 0.0 | 54.679436 | 32 |
TATCTCG | 22765 | 0.0 | 48.402668 | 39 |
TCGTATG | 26435 | 0.0 | 45.76747 | 43 |
TTCTGCT | 22425 | 0.0 | 45.6442 | 55 |
TGTCGTA | 1710 | 0.0 | 45.594536 | 41 |
TGAAAAA | 27425 | 0.0 | 44.31709 | 62 |